| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon-inducible protein AIM2;Absent in melanoma 2;AIM2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AIM2 recombinant protein (Position: L14-H215). Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects interferon-inducible protein AIM2 in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 39.0 kDa, and the supplier reports an observed band at about 45 kDa.
AIM2 (absent in melanoma 2) is an interferon-inducible sensor that detects double-stranded DNA in the cytosol. On binding DNA it assembles an inflammasome with ASC (PYCARD) and caspase-1, leading to IL-1β and IL-18 maturation and pyroptosis, so it is studied in antiviral and antibacterial immunity and autoinflammation.
| Target | Interferon-inducible protein AIM2 (gene AIM2; UniProt O14862, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 39.0 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
Samples with a confirmed band (WB): human A549 cells, human Daudi cells, human K562 cells, human HepG2 cells, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat intestine tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human AIM2 fragment (Leu14–His215), expressed in E. coli. Sequence identity with the mouse ortholog: 53.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places interferon-inducible protein AIM2 in the cytoplasm, inflammasome and nucleus. Tissue expression noted by UniProt (human): Expressed in spleen, small intestine, peripheral blood leukocytes, and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chen Rui et al. (2025) Endothelial HMGB1-AIM2 axis worsens myocardial ischemia—reperfusion injury by regulating endothelial pyroptosis. Apoptosis. 10.1007/s10495-025-02162-5
Limin Chao et al. (2025) Qingqiao polyphenols improve DSS induced ulcerative colitis in mice by inhibiting NLRP3/AIM2 inflammasome mediated pyroptosis. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120480
Pronin Alexey et al. (2019) Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury. Frontiers in Molecular Neuroscience. 10.3389/fnmol.2019.00036
