| Field | Specification |
|---|---|
| Target | |
| Alternative names | Autoimmune regulator;Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein;APECED protein;AIRE;APECED; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AIRE recombinant protein (Position: M1-K120). Human AIRE shares 87.2% amino acid (aa) sequence identity with mouse AIRE. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects autoimmune regulator (gene AIRE) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 57.7 kDa, and the supplier reports an observed band at about 58–68 kDa.
The autoimmune regulator (AIRE) is a transcriptional regulator expressed in medullary thymic epithelial cells. It drives expression of many tissue-restricted self-antigens in the thymus so that self-reactive T cells can be eliminated, and loss of AIRE causes the autoimmune syndrome APECED (APS-1).
| Target | Autoimmune regulator (gene AIRE; UniProt O43918, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 57.7 kDa |
| Observed MW | 58–68 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human U937 cells lysate, human RT4 cells lysate, human Jurkat cells lysate, human K562 cells lysate, rat spleen tissue lysates, rat thymus tissue lysates, mouse spleen tissue lysates, mouse thymus tissue lysates.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human AIRE fragment (Met1–Lys120), expressed in E. coli. Sequence identity with the mouse ortholog: 87.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places autoimmune regulator in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
