| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adenylate kinase 2, mitochondrial ;AK 2 ;2.7.4.3 ;ATP-AMP transphosphorylase 2 ;ATP:AMP phosphotransferase ;Adenylate monophosphate kinase ;Adenylate kinase 2, mitochondrial, N-terminally processed ;AK2 ;ADK2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human AK2 recombinant protein (Position: E161-I239). Human AK2 shares 93.7% and 93% amino acid (aa) sequence identity with mouse and rat AK2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets adenylate kinase 2, mitochondrial (gene AK2) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 26.5 kDa, and the supplier reports an observed band at about 26 kDa.
Adenylate kinase 2 (AK2) is located in the mitochondrial intermembrane space, where it interconverts ATP, ADP and AMP to maintain adenine nucleotide balance. AK2 is required for normal blood cell development, and its deficiency causes reticular dysgenesis, a severe form of combined immunodeficiency.
| Target | Adenylate kinase 2, mitochondrial (gene AK2; UniProt P54819, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 26.5 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human HL-60 cells, human 293T cells, human HepG2 cells, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat liver tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Immunogen
Recombinant human AK2 fragment (Glu161–Ile239), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.7% and 93%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places adenylate kinase 2, mitochondrial in the mitochondrion intermembrane space. Tissue expression noted by UniProt (human): Present in most tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
