| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adenylate kinase 2, mitochondrial; AK 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; AK2; ADK2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AK2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29A42 is a rabbit monoclonal antibody that recognizes adenylate kinase 2, mitochondrial (gene AK2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 26.5 kDa, and the supplier reports an observed band at about 26 kDa.
Adenylate kinase 2 (AK2) is located in the mitochondrial intermembrane space, where it interconverts ATP, ADP and AMP to maintain adenine nucleotide balance. AK2 is required for normal blood cell development, and its deficiency causes reticular dysgenesis, a severe form of combined immunodeficiency.
| Target | Adenylate kinase 2, mitochondrial (gene AK2; UniProt P54819, human) |
|---|---|
| Clone | 29A42 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 26.5 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human AK2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places adenylate kinase 2, mitochondrial in the mitochondrion intermembrane space. Tissue expression noted by UniProt (human): Present in most tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
