| Field | Specification |
|---|---|
| Target | |
| Alternative names | A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95; AKAP8; AKAP95 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AKAP 95 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29A07 is a rabbit monoclonal antibody that recognizes A-kinase anchor protein 8 (gene AKAP8) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 76.1 kDa, and the supplier reports an observed band at about 95 kDa.
AKAP8 (AKAP95) is a nuclear A-kinase anchoring protein that binds type II PKA. During mitosis it helps keep chromosomes condensed by recruiting condensin components, and it has additional roles in cell cycle progression and gene expression.
| Target | A-kinase anchor protein 8 (gene AKAP8; UniProt O43823, human) |
|---|---|
| Clone | 29A07 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 76.1 kDa |
| Observed MW | 95 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human AKAP 95.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places A-kinase anchor protein 8 in the nucleus, nucleus matrix and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
