| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aldose reductase;AR;1.1.1.21;Aldehyde reductase;Aldo-keto reductase family 1 member B1;AKR1B1;ALDR1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human AKR1B1 recombinant protein (Position: L228-F316). Human AKR1B1 shares 87.5% amino acid (aa) sequence identity with both mouse and rat AKR1B1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AKR1B1 (Aldose reductase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-AKR1B1 Antibody Picoband® catalog # PB10035. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human AKR1B1 recombinant protein (Position: L228-F316). Human AKR1B1 shares 87.5% amino acid (aa) sequence identity with both mouse and rat AKR1B1. (reported region: L228-F316).
- Molecular weight context: reported MW: 36 kDa; calculated MW: 35853 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Aldose reductase; Aldose reductase. Aldo-keto reductase family 1, member B1 (aldose reductase), also known as AR, is an enzyme that in humans is encoded by the AKR1B1 gene. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number ofaldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Functional note: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Reported localization: Cytoplasm. Expression/tissue context: Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. .
Research relevance and current trends
- Cancer: Researchers commonly examine how AKR1B1 (Aldose reductase) relates to this theme using model systems and orthogonal readouts.
- Energy Metabolism: Researchers commonly examine how AKR1B1 (Aldose reductase) relates to this theme using model systems and orthogonal readouts.
- Energy Transfer Pathways: Researchers commonly examine how AKR1B1 (Aldose reductase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AKR1B1 (Aldose reductase) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of AKR1B1 (Aldose reductase) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
