| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aldose reductase;AR;1.1.1.21;Aldehyde reductase;Aldo-keto reductase family 1 member B1;AKR1B1;ALDR1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human AKR1B1 recombinant protein (Position: L228-F316). Human AKR1B1 shares 87.5% amino acid (aa) sequence identity with both mouse and rat AKR1B1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets aldo-keto reductase family 1 member B1 (gene AKR1B1) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 35.9 kDa, and the supplier reports an observed band at about 36 kDa.
Aldose reductase (AKR1B1) is an NADPH-dependent aldo-keto reductase that reduces many aldehydes and ketones, including lipid-derived aldehydes. It also reduces glucose to sorbitol, which opens the polyol pathway, which becomes active at high glucose levels and is studied in diabetic complications.
| Target | Aldo-keto reductase family 1 member B1 (gene AKR1B1; UniProt P15121, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 35.9 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human SiHa cells, rat testis tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat intestine tissue, rat adrenal gland tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human AKR1B1 fragment (Leu228–Phe316), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 87.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places aldo-keto reductase family 1 member B1 in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hong-Tao Liu et al. (2023) Spatially resolved transcriptomics revealed local invasion-related genes in colorectal cancer. Frontiers in Oncology. 10.3389/fonc.2023.1089090
