| Field | Specification |
|---|---|
| Target | |
| Alternative names | Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AKT1/2 |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17A25 is a rabbit monoclonal antibody that recognizes AKT1/2 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry.
| Target | AKT1/2 (UniProt P31749/P31751) |
|---|---|
| Clone | 17A25 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Observed MW | 56 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human AKT1/2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fan Qiu et al. (2019) Mulberry Bark Alleviates Effect of STZ Inducing Diabetic Mice through Negatively Regulating FoxO1. Evidence-based Complementary and Alternative Medicine. 10.1155/2019/2182865
Yujing Huang et al. (2017) Effect of hippocampal L‑NBP on BDNF and TrkB expression and neurological function of vascular dementia rats. Molecular Medicine Reports. 10.3892/mmr.2017.7539
Jun Wan et al. (2015) SOCS3 blocks HIF-1α expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3. Molecular Medicine Reports. 10.3892/mmr.2015.3368
