Anti-AKT1 + AKT2 + AKT3 Rabbit Monoclonal Antibody

SKU:BHA21010614
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-AKT1 antibody (Rabbit monoclonal, clone 31A24, IgG). Validated for Western blot, immunohistochemistry, immunofluorescence, flow cytometry and immunoprecipitation. Reactivity: Human, Mouse, Rat. Supplied in liquid form, ready for use in standard immunodetection workflows.
Target AKT1
clone number 31A24
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB, IHC, IF, ICC, FC, IP
Options selector
Catalog no. Size Conjugation
M00024-7 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB,IHC,ICC,IF,IP,Flow Cytometry
      • Application details: WB 1:500-2000
        IHC 1:50-200
        ICC/IF 1:50-200
        IP 1:50
        FC 1:50
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target AKT1
UniProt # P31749/P31751/Q9Y243
Host Rabbit
Clone 31A24
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human AKT1 + AKT2 + AKT3
Purification Affinity-chromatography
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00024-7
Main SKU BHA21010614
Rabbit Monoclonal

Product Overview

Clone 31A24 is a rabbit monoclonal antibody that recognizes AKT1 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and immunoprecipitation.

Target AKT1 (UniProt P31749/P31751/Q9Y243)
Clone 31A24
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Purification Affinity-chromatography
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Concentration 0.5 mg/mL
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–2000 IHC 1:50–200 ICC/IF 1:50–200 IP 1:50 FC 1:50
Immunohistochemistry Not specified by the supplier
Immunocytochemistry / Immunofluorescence Not specified by the supplier
Flow cytometry Not specified by the supplier
Immunoprecipitation Not specified by the supplier

Immunogen

Synthetic peptide based on the sequence of human AKT1 + AKT2 + AKT3.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–2000 IHC 1:50–200 ICC/IF 1:50–200 IP 1:50 FC 1:50; Immunohistochemistry (dilution not specified); Immunocytochemistry / Immunofluorescence (dilution not specified); Flow cytometry (dilution not specified); Immunoprecipitation (dilution not specified). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human AKT1 + AKT2 + AKT3.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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