| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; RAC |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EBG-1 is a rabbit monoclonal antibody that recognizes RAC-alpha serine/threonine-protein kinase (gene AKT1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 55.7 kDa, and the supplier reports an observed band at about 56 kDa.
AKT1 (protein kinase Bα) is one of three AKT serine/threonine kinases activated downstream of PI3K by growth factors and insulin. By phosphorylating many substrates, it promotes cell survival, growth, proliferation, glucose uptake and angiogenesis, and it is one of the most studied nodes in cancer and metabolic signaling.
| Target | RAC-alpha serine/threonine-protein kinase (gene AKT1; UniProt P31749, human) |
|---|---|
| Clone | EBG-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.7 kDa |
| Observed MW | 56 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human A549 cells, human HeLa cells, human Raji cells, human MCF-7 cells, human SiHa cells, human 293T cells, rat liver tissue, rat brain tissue, rat C6 cells, rat RH35 cells, mouse liver tissue, mouse brain tissue, mouse Neuro-2a cells, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places RAC-alpha serine/threonine-protein kinase in the cytoplasm, nucleus, cell membrane and mitochondrion intermembrane space. Tissue expression noted by UniProt (human): Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Aslıhan ŞENGELEN et al. (2024) Rosmarinic acid attenuates glioblastoma cells and spheroids’ growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis. Phytomedicine. 10.1016/j.phymed.2024.156060
Han Chang et al. (2021) Cichorium pumilum Jacq Extract Inhibits LPS-Induced Inflammation via MAPK Signaling Pathway and Protects Rats From Hepatic Fibrosis Caused by Abnormalities in the Gut-Liver Axis. Frontiers in Pharmacology. 10.3389/fphar.2021.683613
Evren Önay Uçar et al. (2023) Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells. Biochemical Pharmacology. 10.1016/j.bcp.2022.115409
Ying Zhao et al. (2022) G protein-coupled receptor 30 mediates cell proliferation of goat mammary epithelial cells via MEK/ERK&PI3K/AKT signaling pathway. Cell Cycle. 10.1080/15384101.2022.2083708
Na Yang et al. (2025) Bioactive compound combinations from Rhodiola tangutica alleviate pulmonary vascular remodeling in high-altitude pulmonary hypertension rats through the PI3K–AKT pathway. Frontiers in Pharmacology. 10.3389/fphar.2025.1582677
Yeke Wu et al. (2024) A breakthrough in periodontitis treatment: Revealing the pharmacodynamic substances and mechanisms of Kouqiangjie formula. Journal of Ethnopharmacology. 10.1016/j.jep.2024.117738
Wen-Jie Ma et al. (2023) PI3K-CCL2-CCR2-MDSCs axis: A potential pathway for tumor Clostridia-promoted CD 8+ T lymphocyte infiltration in bile tract cancers. Neoplasia. 10.1016/j.neo.2023.100920
Bin Hou et al. (2025) Luteolin alleviates right ventricular hypertrophy in high altitude pulmonary hypertension rats by regulating PI3K/AKT/mTOR signalling pathway. European Journal of Pharmacology. 10.1016/j.ejphar.2025.177898
Li Rui et al. (2017) Metabolic syndrome in rats is associated with erectile dysfunction by impairing PI3K/Akt/eNOS activity. Scientific Reports. 10.1038/s41598-017-12907-1
ZiQian Liu et al. (2026) YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR–HIF1α–LHDA Axis. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.71105
