| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAC-beta serine/threonine-protein kinase;2.7.11.1;Protein kinase Akt-2;Protein kinase B beta;PKB beta;RAC-PK-beta;AKT2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human AKT2, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects RAC-beta serine/threonine-protein kinase (gene AKT2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 55.8 kDa, and the supplier reports an observed band at about 56 kDa.
AKT2 (protein kinase Bβ) is an AKT family serine/threonine kinase activated through the PI3K pathway. It is especially important in insulin signaling, where it drives GLUT4 translocation and glucose uptake in muscle and fat, and it also supports cell survival and proliferation, making it a focus of diabetes and cancer research.
| Target | RAC-beta serine/threonine-protein kinase (gene AKT2; UniProt P31751, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 55.8 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human SW620 cells, human 293T cells, human MCF-7 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): rat brain tissue.
Immunogen
Synthetic peptide from the C-terminal region of human AKT2. It differs from the mouse sequence at two residues and from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places RAC-beta serine/threonine-protein kinase in the cytoplasm, nucleus, cell membrane and early endosome. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Liu et al. (2015) Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model. International Journal of Clinical and Experimental Medicine. PMID: 26550236
