| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aldehyde dehydrogenase, mitochondrial;1.2.1.3;ALDH class 2;ALDH-E2;ALDHI;ALDH2;ALDM; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ALDH2, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects aldehyde dehydrogenase, mitochondrial (gene ALDH2) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 56.4 kDa, and the supplier reports an observed band at about 56 kDa.
ALDH2 is the mitochondrial aldehyde dehydrogenase chiefly responsible for oxidizing acetaldehyde produced from ethanol, and it also removes aldehydes from lipid peroxidation. The common ALDH2*2 variant in East Asian populations reduces activity and causes alcohol flushing, so ALDH2 is studied in alcohol metabolism, oxidative stress and cardiovascular protection.
| Target | Aldehyde dehydrogenase, mitochondrial (gene ALDH2; UniProt P05091, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 56.4 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, rat liver tissue, rat lung tissue, rat kidney tissue, mouse kidney tissue, mouse ANA-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Synthetic peptide from the N-terminal region of human ALDH2. It differs from the mouse sequence at two residues and from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places aldehyde dehydrogenase, mitochondrial in the mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Yiqiu Li et al. (2020) Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways. Cancers. 10.3390/cancers12010138
Fengming Tian et al. (2023) Integrating network pharmacology and bioinformatics to explore and experimentally verify the regulatory effect of Buyang Huanwu decoction on glycolysis and angiogenesis after cerebral infarction. Journal of Ethnopharmacology. 10.1016/j.jep.2023.117218
Xuebing Cheng et al. (2020) Bioinformatic and biochemical findings disclosed anti-hepatic steatosis mechanism of calycosin. Bioorganic Chemistry. 10.1016/j.bioorg.2020.103914
Luo Nuanyuan et al. (2020) Trichloroethylene injures rat liver and elevates the level of peroxisomal bifunctional enzyme (Ehhadh). Molecular & Cellular Toxicology. 10.1007/s13273-020-00075-9
