| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aldehyde dehydrogenase, mitochondrial; ALDH I; ALDH class 2; ALDH-2; ALDH-E2; Nitroglycerin reductase ALDH2; ALDH2; ALDM |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ALDH2 Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFOI-1 is a rabbit monoclonal antibody that recognizes aldehyde dehydrogenase, mitochondrial (gene ALDH2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 56.4 kDa, and the supplier reports an observed band at about 140 kDa.
ALDH2 is the mitochondrial aldehyde dehydrogenase chiefly responsible for oxidizing acetaldehyde produced from ethanol, and it also removes aldehydes from lipid peroxidation. The common ALDH2*2 variant in East Asian populations reduces activity and causes alcohol flushing, so ALDH2 is studied in alcohol metabolism, oxidative stress and cardiovascular protection.
| Target | Aldehyde dehydrogenase, mitochondrial (gene ALDH2; UniProt P05091, human) |
|---|---|
| Clone | AFOI-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.4 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human ALDH2 Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places aldehyde dehydrogenase, mitochondrial in the mitochondrion.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
