| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fatty aldehyde dehydrogenase;1.2.1.3;Aldehyde dehydrogenase 10;Aldehyde dehydrogenase family 3 member A2;Microsomal aldehyde dehydrogenase;ALDH3A2;ALDH10, FALDH; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human ALDH3A2 recombinant protein (Position: M1-Q100). Human ALDH3A2 shares 78% amino acid (aa) sequence identity with both mouse and rat ALDH3A2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ALDH3A2 (Fatty aldehyde dehydrogenase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ALDH3A2 Antibody Picoband® catalog # PB9982. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human ALDH3A2 recombinant protein (Position: M1-Q100). Human ALDH3A2 shares 78% amino acid (aa) sequence identity with both mouse and rat ALDH3A2. (reported region: M1-Q100).
- Molecular weight context: reported MW: 55 kDa; calculated MW: 54848 MW
- Reactivity: Human,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Fatty aldehyde dehydrogenase; Fatty aldehyde dehydrogenase. Fatty aldehyde dehydrogenase (or Long-chain-aldehyde dehydrogenase) is an aldehyde dehydrogenase enzyme that in human is encoded in the ALDH3A2 gene on chromosome 17. ALDH3A2 catalyzes the oxidation of long-chain aliphatic aldehydes into fatty acids. It is known to act on a variety of both saturated and unsaturated aliphatic aldehydes between 6 to 24 carbons in length, as well as dihydrophytal, a 20-carbon branched chain aldehyde. It requires NAD+ as a co-factor. The encoded enzyme is responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. ALD3H2 is expressed in the human liver and has been found to localize the microsome fraction inside the cell. Functional note: Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. . Reported localization: Endoplasmic reticulum membrane ; Single-pass membrane protein ; Cytoplasmic side . Expression/tissue context: Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.
Research relevance and current trends
- Developmental Biology: Researchers commonly examine how ALDH3A2 (Fatty aldehyde dehydrogenase) relates to this theme using model systems and orthogonal readouts.
- Nervous System Development: Researchers commonly examine how ALDH3A2 (Fatty aldehyde dehydrogenase) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how ALDH3A2 (Fatty aldehyde dehydrogenase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ALDH3A2 (Fatty aldehyde dehydrogenase) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ALDH3A2 (Fatty aldehyde dehydrogenase) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
