| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fatty aldehyde dehydrogenase;1.2.1.3;Aldehyde dehydrogenase 10;Aldehyde dehydrogenase family 3 member A2;Microsomal aldehyde dehydrogenase;ALDH3A2;ALDH10, FALDH; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ALDH3A2 recombinant protein (Position: M1-Q100). Human ALDH3A2 shares 78% amino acid (aa) sequence identity with both mouse and rat ALDH3A2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects aldehyde dehydrogenase family 3 member A2 (gene ALDH3A2) in human and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 54.8 kDa, and the supplier reports an observed band at about 55 kDa.
ALDH3A2 (fatty aldehyde dehydrogenase) oxidizes medium- and long-chain fatty aldehydes to fatty acids, including hexadecenal released during sphingosine 1-phosphate breakdown. Loss-of-function mutations cause Sjögren-Larsson syndrome, a disorder affecting skin and the nervous system.
| Target | Aldehyde dehydrogenase family 3 member A2 (gene ALDH3A2; UniProt P51648, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 54.8 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): rat liver tissue, rat testis tissue, human HeLa cells, human A431 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ALDH3A2 fragment (Met1–Gln100), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 78%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places aldehyde dehydrogenase family 3 member A2 in the microsome membrane and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Detected in liver (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
