| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-aminoadipic semialdehyde dehydrogenase;Alpha-AASA dehydrogenase;1.2.1.31;Aldehyde dehydrogenase family 7 member A1;1.2.1.3;Antiquitin-1;Betaine aldehyde dehydrogenase;1.2.1.8;Delta1-piperideine-6-carboxylate dehydrogenase;P6c dehydrogenase;ALDH7A1;ATQ1; |
| UniProt # | |
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| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ALDH7A1, different from the related mouse sequence by eight amino acids, and from the related rat sequence by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
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| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody recognizes alpha-aminoadipic semialdehyde dehydrogenase (gene ALDH7A1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 58.5 kDa, and the supplier reports an observed band at about 55 kDa.
ALDH7A1 (antiquitin) is an aldehyde dehydrogenase that oxidizes α-aminoadipic semialdehyde in lysine breakdown and also converts betaine aldehyde to the osmolyte betaine. Mutations cause pyridoxine-dependent epilepsy, and the enzyme is also studied for its role in protecting cells from lipid peroxidation-derived aldehydes.
| Target | Alpha-aminoadipic semialdehyde dehydrogenase (gene ALDH7A1; UniProt P49419, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 58.5 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human hccp tissue, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat brain tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Synthetic peptide from the C-terminal region of human ALDH7A1. It differs from the mouse sequence at eight residues and from the rat sequence at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Abundant in hepatoma cells and fetal cochlea, ovary, eye, heart, adrenal gland, liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lingling Geng et al. (2025) Systematic profiling reveals betaine as an exercise mimetic for geroprotection. Cell. 10.1016/j.cell.2025.06.001
