| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDOA; ALDA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Aldolase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEAB-1 is a rabbit monoclonal antibody that recognizes fructose-bisphosphate aldolase A (gene ALDOA) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 39.4 kDa, and the supplier reports an observed band at about 39 kDa.
Aldolase A (ALDOA) is the glycolytic enzyme that reversibly splits fructose 1,6-bisphosphate into two triose phosphates, and it is the main aldolase in muscle and many other tissues. Beyond glycolysis, it binds actin and can act as a glucose sensor that links low glucose to AMPK activation.
| Target | Fructose-bisphosphate aldolase A (gene ALDOA; UniProt P04075, human) |
|---|---|
| Clone | AEAB-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.4 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human aldolase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places fructose-bisphosphate aldolase A in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
