| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95; PDCD6IP; AIP1; ALIX; KIAA1375 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ALIX |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEBO-16 is a rabbit monoclonal antibody that recognizes programmed cell death 6-interacting protein (gene PDCD6IP) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 96.0 kDa, and the supplier reports an observed band at about 96 kDa.
ALIX (PDCD6IP) is a multifunctional adaptor of the ESCRT machinery. It links cargo sorting at endosomes to ESCRT-III proteins such as CHMP4, supporting formation of intralumenal vesicles in multivesicular bodies, exosome release and the final abscission step of cytokinesis. Because of these roles, ALIX is a widely used exosome marker and is studied in membrane repair, virus budding and apoptosis.
| Target | Programmed cell death 6-interacting protein (gene PDCD6IP; UniProt Q8WUM4, human) |
|---|---|
| Clone | AEBO-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 96.0 kDa |
| Observed MW | 96 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human K562 cells, human Raji cells, human Daudi cells, rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ALIX.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes programmed cell death 6-interacting protein as secreted and also located in the cytoplasm, melanosome and cell junction. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shuangqin Chen et al. (2022) β-catenin-controlled tubular cell-derived exosomes play a key role in fibroblast activation via the OPN-CD44 axis. Journal of Extracellular Vesicles. 10.1002/jev2.12203
Edita Aksamitiene et al. (2025) Defining Biological Variability, Analytical Precision and Quantitative Biophysiochemical Characterization of Human Urinary Extracellular Vesicles. Journal of Extracellular Vesicles. 10.1002/jev2.70087
Alexander M Fliflet et al. (2026) Exercise Training Stimulates the Release of Glutathione Peroxidase 1 (GPX1)‐Enriched Extracellular Vesicles That Promote Angiogenesis. FASEB Journal. 10.1096/fj.202505096RR
Sorrells Janet E. et al. (2024) Label-free nonlinear optical signatures of extracellular vesicles in liquid and tissue biopsies of human breast cancer. Scientific Reports. 10.1038/s41598-024-55781-4
