Anti-Alkaline phosphatase ALPI Rabbit Monoclonal Antibody

Overview

This product is an anti-ALPI antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEGC-1; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-Alkaline phosphatase ALPI Rabbit Monoclonal Antibody catalog # M06115. Tested in WB, IHC applications. This antibody reacts with Human.

Key elements and design rationale

• Target: ALPI (Flotillin-2).
• Antibody format: Monoclonal; clone AEGC-1; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

ALPI (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. Reported cellular localization context: Cell membrane ; Peripheral membrane protein . Membrane, caveola ; Peripheral membrane protein . Endosome . Membrane-associated protein of caveolae. Tissue expression notes (as provided): In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cell Biology,Cell Cycle,Kinases/Phosphatases,Tags & Cell Markers,Tumor Biomarkers.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.

Workflow ideas (metafield): Validate ALPI antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ALPI expression by Western blot in cell or tissue lysates, Detect ALPI in FFPE tissue sections by immunohistochemistry

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 25 kDa; calculated MW: 47064 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 25 kDa
• Cellular localization (provided): Cell membrane ; Peripheral membrane protein . Membrane, caveola ; Peripheral membrane protein . Endosome . Membrane-associated protein of caveolae.
• Tissue details (provided): In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.