| Field | Specification |
|---|---|
| Target | |
| Alternative names | Intestinal-type alkaline phosphatase; IAP; Intestinal alkaline phosphatase; ALPI |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human intestinal alkaline phosphatase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEGC-1 is a rabbit monoclonal antibody that recognizes intestinal-type alkaline phosphatase (gene ALPI) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 56.8 kDa, and the supplier reports an observed band at about 68 kDa.
Intestinal alkaline phosphatase (ALPI) is a brush border enzyme of the small intestine that hydrolyzes phosphate esters. It is thought to help maintain gut homeostasis, for example by dephosphorylating bacterial lipopolysaccharide, and it is studied in intestinal barrier function and inflammation.
| Target | Intestinal-type alkaline phosphatase (gene ALPI; UniProt P09923, human) |
|---|---|
| Clone | AEGC-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.8 kDa |
| Observed MW | 68 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human intestinal alkaline phosphatase.
Reactivity Notes
The supplier lists reactivity with human. UniProt places intestinal-type alkaline phosphatase in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
