| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNS-ALP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; Phosphoamidase; Phosphocreatine phosphatase; ALPL |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Alkaline phosphatase/ALPL recombinant protein (Position: K229-H482). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes alkaline phosphatase, tissue-nonspecific isozyme (gene ALPL) in human, mouse and rat samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 57.3 kDa, and the supplier reports an observed band at about 80 kDa.
Tissue-nonspecific alkaline phosphatase (TNAP, ALPL) is expressed in bone, liver and kidney. By hydrolyzing inorganic pyrophosphate, an inhibitor of mineralization, and pyridoxal 5′-phosphate, it is essential for bone and tooth mineralization, and ALPL mutations cause hypophosphatasia.
| Target | Alkaline phosphatase, tissue-nonspecific isozyme (gene ALPL; UniProt P05186, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 57.3 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HEK293 cells, rat kidney tissue, rat RH-35 cells, mouse kidney tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:100, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Recombinant human Alkaline phosphatase/ALPL fragment (Lys229–His482), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alkaline phosphatase, tissue-nonspecific isozyme in the cell membrane, extracellular vesicle membrane, mitochondrion membrane and mitochondrion intermembrane space. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenhao Cheng et al. (2026) Black Phosphorus Nanosheets for Enhanced Osteogenic Differentiation of Dental Follicle Stem Cells. ACS Applied Nano Materials. 10.1021/acsanm.6c00876
Chao Shan et al. (2024) Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling. International Immunopharmacology. 10.1016/j.intimp.2024.112299
