| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alkaline phosphatase, tissue-nonspecific isozyme;AP-TNAP;TNSALP;3.1.3.1 ;Alkaline phosphatase liver/bone/kidney isozyme;ALPL; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related rat and mouse sequences by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Alkaline Phosphatase/ALPL Antibody Picoband® is an antibody targeting ALPL. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 80 kDa; calculated MW: 57305 MW.
Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: ALPL — Alkaline phosphatase, tissue-nonspecific isozyme
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 80 kDa; Calculated: 57305 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): This isozyme may play a role in skeletal mineralization.
Scientific background (datasheet): Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Cellular localization (datasheet): Cell membrane ; Lipid-anchor, GPI-anchor .
Tissue details (datasheet): Widely expressed, with highest expression in brain. .
Sequence similarities (datasheet): Contains 12 fibronectin type-I domains.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cell Biology,Cell Cycle,Kinases/Phosphatases,Tags & Cell Markers,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
