| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alkaline phosphatase, tissue-nonspecific isozyme;AP-TNAP;TNSALP;3.1.3.1 ;Alkaline phosphatase liver/bone/kidney isozyme;ALPL; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets alkaline phosphatase, tissue-nonspecific isozyme (gene ALPL) in human samples and is validated for Western blot. The predicted molecular weight is 57.3 kDa, and the supplier reports an observed band at about 57 kDa.
Tissue-nonspecific alkaline phosphatase (TNAP, ALPL) is expressed in bone, liver and kidney. By hydrolyzing inorganic pyrophosphate, an inhibitor of mineralization, and pyridoxal 5′-phosphate, it is essential for bone and tooth mineralization, and ALPL mutations cause hypophosphatasia.
| Target | Alkaline phosphatase, tissue-nonspecific isozyme (gene ALPL; UniProt P05186, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 57.3 kDa |
| Observed MW | 57 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human placenta tissue, HT1080 cells, Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of human alkaline Phosphatase. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places alkaline phosphatase, tissue-nonspecific isozyme in the cell membrane, extracellular vesicle membrane, mitochondrion membrane and mitochondrion intermembrane space. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
