| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alkaline phosphatase, tissue-nonspecific isozyme;AP-TNAP;TNSALP;3.1.3.1 ;Alkaline phosphatase liver/bone/kidney isozyme;ALPL; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Alkaline Phosphatase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DBF-1 is a rabbit monoclonal antibody that recognizes alkaline phosphatase, tissue-nonspecific isozyme (gene ALPL) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 57.3 kDa, and the supplier reports an observed band at about 80 kDa.
Tissue-nonspecific alkaline phosphatase (TNAP, ALPL) is expressed in bone, liver and kidney. By hydrolyzing inorganic pyrophosphate, an inhibitor of mineralization, and pyridoxal 5′-phosphate, it is essential for bone and tooth mineralization, and ALPL mutations cause hypophosphatasia.
| Target | Alkaline phosphatase, tissue-nonspecific isozyme (gene ALPL; UniProt P05186, human) |
|---|---|
| Clone | DBF-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.3 kDa |
| Observed MW | 80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human Jurkat cells, human MCF-7 cells, human K562 cells, rat liver tissue, rat spleen tissue, mouse liver tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:5000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human alkaline Phosphatase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alkaline phosphatase, tissue-nonspecific isozyme in the cell membrane, extracellular vesicle membrane, mitochondrion membrane and mitochondrion intermembrane space. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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