Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody

Overview

This product is an anti-ALPL antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone DBF-1; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody catalog # M01008-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: ALPL (Alkaline phosphatase, tissue-nonspecific isozyme).
• Antibody format: Monoclonal; clone DBF-1; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

ALPL (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): This isozyme may play a role in skeletal mineralization. Reported cellular localization context: Cell membrane ; Lipid-anchor, GPI-anchor . Tissue expression notes (as provided): Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cell Biology,Cell Cycle,Kinases/Phosphatases,Tags & Cell Markers,Tumor Biomarkers.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate ALPL antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ALPL expression by Western blot in cell or tissue lysates, Detect ALPL in FFPE tissue sections by immunohistochemistry, Localize ALPL by immunofluorescence/immunocytochemistry in cultured cells, Quantify ALPL-positive cells by flow cytometry in single-cell suspensions, Enrich ALPL by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 80 kDa; calculated MW: 57305 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 80 kDa
• Cellular localization (provided): Cell membrane ; Lipid-anchor, GPI-anchor .
• Tissue details (provided): Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.