| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleic acid dioxygenase ALKBH1; Alkylated DNA repair protein alkB homolog 1; Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA 6mA demethylase; DNA N6-methyl adenine demethylase ALKBH1; DNA lyase ABH1; DNA oxidative demethylase ALKBH1; mRNA N(3)-methylcytidine demethylase; ALKBH1; ABH; ABH1; ALKBH |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ALKBH1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25A31 is a rabbit monoclonal antibody that recognizes nucleic acid dioxygenase ALKBH1 in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 43.8 kDa, and the supplier reports an observed band at about 44 kDa.
ALKBH1 is an Fe(II)/α-ketoglutarate-dependent dioxygenase of the AlkB family that modifies nucleic acids. It acts mainly on tRNAs, removing N1-methyladenine or oxidizing specific bases in cytoplasmic and mitochondrial tRNAs, and so influences translation and mitochondrial function.
| Target | Nucleic acid dioxygenase ALKBH1 (gene ALKBH1; UniProt Q13686, human) |
|---|---|
| Clone | 25A31 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.8 kDa |
| Observed MW | 44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human ALKBH1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nucleic acid dioxygenase ALKBH1 in the nucleus and mitochondrion. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
