| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-1-antiproteinase;Alpha-1-antitrypsin;Alpha-1-proteinase inhibitor;Serpin A1;Serpina1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat SERPINA1 recombinant protein (Position: K211-R411). Rat SERPINA1 shares 66% amino acid (aa) sequence identity with human SERPINA1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects alpha-1-antiproteinase (gene Serpina1) in mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 46.1 kDa, and the supplier reports an observed band at about 53 kDa.
Alpha-1-antitrypsin (SERPINA1) is an abundant plasma serine protease inhibitor made mainly by the liver, whose main target is neutrophil elastase. By limiting elastase activity it protects lung tissue, and inherited SERPINA1 deficiency variants cause emphysema and liver disease, making it a major subject in respiratory and hepatic research.
| Target | Alpha-1-antiproteinase (gene Serpina1; UniProt P17475, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 46.1 kDa |
| Observed MW | 53 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): rat kidney tissue, rat lung tissue, rat testis tissue, mouse kidney tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat spleen tissue, mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant rat SERPINA1 fragment (Lys211–Arg411), expressed in E. coli. Sequence identity with the human ortholog: 66%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes alpha-1-antiproteinase as a secreted protein. Tissue expression noted by UniProt (rat): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaoping Zhang et al. (2014) Tetramethylpyrazine inhibits angiotensin II-induced activation of hepatic stellate cells associated with interference of platelet-derived growth factor β receptor pathways. FEBS Journal. 10.1111/febs.12818
Fang Jiao et al. (2016) Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection. Journal of Proteomics. 10.1016/j.jprot.2016.08.013
Xiao-Gang Zhong et al. (2007) Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. World Journal of Gastroenterology. 10.3748/wjg.v13.i28.3886
