| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Afp |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Alpha 1 Fetoprotein recombinant protein (Position: K19-L254). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes alpha-fetoprotein (gene Afp) in mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 67.3 kDa, and the supplier reports an observed band at about 69 kDa.
Alpha-fetoprotein (AFP) is the major serum protein of the fetus, produced mainly by the fetal liver and yolk sac and related in structure to albumin. It binds fatty acids, metal ions and bilirubin, and its levels are widely measured in studies of fetal development and liver tumors such as hepatocellular carcinoma.
| Target | Alpha-fetoprotein (gene Afp; UniProt P02772, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 67.3 kDa |
| Observed MW | 69 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse HEPA1–6 cells, rat RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse Alpha 1 Fetoprotein fragment (Lys19–Leu254), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes alpha-fetoprotein as a secreted protein. Tissue expression noted by UniProt (mouse): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yongmin Yan et al. (2009) Mesenchymal stem cells from human umbilical cords ameliorate mouse hepatic injury in vivo. Liver International. 10.1111/j.1478-3231.2008.01855.x
