| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-actinin-2;Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein;ACTN2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Alpha actinin 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21A00 is a rabbit monoclonal antibody that recognizes alpha-actinin-2 (gene ACTN2) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 103.9 kDa, and the supplier reports an observed band at about 103 kDa.
Alpha-actinin-2 (ACTN2) is the main alpha-actinin of cardiac and skeletal muscle. It cross-links actin filaments at the Z-disc and anchors the sarcomere, and variants in ACTN2 are associated with cardiomyopathies.
| Target | Alpha-actinin-2 (gene ACTN2; UniProt P35609, human) |
|---|---|
| Clone | 21A00 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 103.9 kDa |
| Observed MW | 103 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Samples with a confirmed band (WB): human skeletal muscle.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human alpha actinin 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alpha-actinin-2 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in both skeletal and cardiac muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
