| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-soluble NSF attachment protein; SNAP-alpha; N-ethylmaleimide-sensitive factor attachment protein alpha; NAPA; SNAPA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Alpha SNAP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25N48 is a rabbit monoclonal antibody that recognizes alpha-soluble NSF attachment protein (gene NAPA) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 33.2 kDa, and the supplier reports an observed band at about 33 kDa.
α-SNAP (NAPA) is a soluble adaptor that binds assembled SNARE complexes and recruits the ATPase NSF, which disassembles SNAREs after membrane fusion so they can be reused. It is therefore needed for ongoing vesicle traffic, including ER-to-Golgi transport and regulated exocytosis.
| Target | Alpha-soluble NSF attachment protein (gene NAPA; UniProt P54920, human) |
|---|---|
| Clone | 25N48 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.2 kDa |
| Observed MW | 33 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Molt4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human alpha SNAP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alpha-soluble NSF attachment protein in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
