| Field | Specification |
|---|---|
| Target | |
| Alternative names | AP-2 complex subunit alpha-1; 100 kDa coated vesicle protein A; Adaptor protein complex AP-2 subunit alpha-1; Adaptor-related protein complex 2 subunit alpha-1; Alpha-adaptin A; Alpha1-adaptin; Clathrin assembly protein complex 2 alpha-A large chain; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; AP2A1; ADTAA; CLAPA1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Alpha1-adaptin. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFDO-1 is a rabbit monoclonal antibody that recognizes AP-2 complex subunit alpha-1 (gene AP2A1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 107.5 kDa, and the supplier reports an observed band at about 108 kDa.
AP2A1 is one of two α subunits of adaptor protein complex 2 (AP-2), the main clathrin adaptor at the plasma membrane. AP-2 binds cargo and clathrin to build coated pits, and its α appendage recruits accessory proteins needed for clathrin-mediated endocytosis.
| Target | AP-2 complex subunit alpha-1 (gene AP2A1; UniProt O95782, human) |
|---|---|
| Clone | AFDO-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 107.5 kDa |
| Observed MW | 108 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Immunogen
Synthetic peptide based on the sequence of human alpha1-adaptin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places AP-2 complex subunit alpha-1 in the cell membrane and membrane. Tissue expression noted by UniProt (human): Expressed in the brain (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
