Anti-Alphaherpesviruses gB Broad-Neutralizing Recombinant Antibody (16F9)

What it means: Monoclonal describes an antibody derived from a single B-cell clone, so every molecule in the vial recognizes the same epitope. Clone 16F9 here is produced recombinantly, which locks the sequence in place permanently.

Why it matters:

• Monoclonal binding to one defined epitope gives high specificity and low background in quantitative assays.
• Recombinant production removes hybridoma drift, so lot-to-lot performance stays consistent over years of reordering.

How to interpret here: This Monoclonal antibody targets a conserved gB epitope shared across alphaherpesviruses, which is why one clone can neutralize many family members.

Tip: For standardized, multi-year studies a recombinant monoclonal is generally preferred over polyclonal serum.

What it means: The host is the species whose immune repertoire the antibody sequence originates from — here the antibody framework is Mouse, expressed recombinantly in culture.

Why it matters:

• Your secondary antibody must be anti-Mouse IgG to detect this reagent in ELISA, IF, or FCM.
• Mouse framework avoids background when staining non-murine samples such as infected human or veterinary cell lines.

How to interpret here: Pair this Mouse antibody with an anti-mouse conjugated secondary; when working with mouse-derived samples, include isotype and no-primary controls to separate signal from endogenous immunoglobulin.

Tip: For multiplex panels choose other primaries from non-mouse hosts to avoid cross-detection.

What it means: gB (envelope glycoprotein B) is the fusogen of herpesviruses — the class III fusion protein that drives merger of the viral envelope with the host cell membrane during entry.

Why it matters:

• gB is the most conserved envelope glycoprotein across the alphaherpesvirus subfamily, so one well-chosen epitope enables pan-alphaherpesvirus detection.
• Because fusion is essential, antibodies against gB are central tools in neutralization and entry-mechanism studies.

How to interpret here: Clone 16F9 binds a conserved gB epitope, giving broad, neutralizing reactivity from HSV-1/2 and VZV to veterinary herpesviruses.

Tip: Confirm your strain of interest appears in the reactivity list before ordering.

What it means: These are the validated applications for this antibody: ELISA, Biophysics (BLI/SPR/ITC), Immunofluorescence, Flow Cytometry. The vendor has demonstrated performance in each format.

Why it matters:

• SPR-class biophysics validation indicates well-behaved, quantifiable binding kinetics — useful for affinity ranking and epitope binning.
• IF and Flow Cytometry validation means the epitope is accessible on cell-associated antigen, not only on plate-coated protein.

How to interpret here: ELISA is titrated at 1:2000–1:20000; for Immunofluorescence and Flow Cytometry, determine optimal concentration empirically starting from the ELISA range.

Tip: Validation does not transfer between applications — re-titrate whenever you switch formats.

What it means: 16F9 is the clone identifier — the unique B-cell lineage this recombinant monoclonal antibody was cloned from. Every vial labeled 16F9 contains the identical antibody sequence.

Why it matters:

• Citing clone 16F9 in publications lets other laboratories reproduce your work with the exact same reagent.
• A defined clone number guarantees epitope consistency across lots, which polyclonal antisera cannot offer.

How to interpret here: 16F9 was selected for broad neutralizing reactivity against conserved gB, so it behaves consistently across the listed alphaherpesviruses.

Tip: Record the clone number, not just the catalog number, in your methods section.

What it means: Viral/Pathogen reactivity indicates this antibody recognizes a pathogen-encoded antigen — here the gB glycoprotein carried by alphaherpesviruses — rather than a host-species protein.

Why it matters:

• Species compatibility works differently for pathogen antibodies: what matters is the virus strain, not the host organism of your sample.
• Sequence conservation of the epitope across viral strains determines breadth — this clone spans eleven alphaherpesviruses.

How to interpret here: With Viral/Pathogen reactivity, verify your working strain appears in the detailed reactivity list (HSV-1, HSV-2, VZV, PRV, BHV-1/2/5, CpHV-1, CHV-1, FHV-1, McHV-1).

Tip: For unlisted strains, run a pilot titration before committing a full study.