| Field | Specification |
|---|---|
| Target | |
| Alternative names | NIF3-like protein 1; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 1 protein; NIF3L1; ALS2CR1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ALS2CR1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28N08 is a rabbit monoclonal antibody that recognizes NIF3-like protein 1 (gene NIF3L1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 42.0 kDa, and the supplier reports an observed band at about 38 kDa.
NIF3L1 is a protein conserved from bacteria to humans, related to yeast NIF3. It has been reported to act as a transcriptional corepressor through the COP9 signalosome subunit COPS2, dampening genes involved in neuronal differentiation, but its function is not fully characterized.
| Target | NIF3-like protein 1 (gene NIF3L1; UniProt Q9GZT8, human) |
|---|---|
| Clone | 28N08 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.0 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): LNCaP cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ALS2CR1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places NIF3-like protein 1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
