| Field | Specification |
|---|---|
| Target | |
| Alternative names | AMPD1; AMP deaminase 1; EC 3.5.4.6; AMP deaminase isoform M; Myoadenylate deaminase |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AMPD1 recombinant protein (Position: K5-E747). Human AMPD1 shares 92.7% amino acid (aa) sequence identity with both mouse and rat AMPD1. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes AMP deaminase 1 (gene AMPD1) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
AMP deaminase 1 (AMPD1) is the skeletal muscle isoform of AMP deaminase, which converts AMP to IMP and ammonia. This reaction is part of the purine nucleotide cycle and helps keep the energy charge high during intense exercise; AMPD1 deficiency is a common inherited metabolic variant.
| Target | AMP deaminase 1 (gene AMPD1; UniProt P23109, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human AMPD1 fragment (Lys5–Glu747), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 92.7%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
