| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AMPK alpha 1/PRKAA1 recombinant protein (Position: D359-P539). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes 5'-AMP-activated protein kinase catalytic subunit alpha-1 (gene PRKAA1) in human, monkey, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 64.0 kDa, and the supplier reports an observed band at about 64–66 kDa.
AMPK α1 (PRKAA1) is one of the two catalytic subunits of AMP-activated protein kinase, the cell's main energy sensor. When ATP falls, AMPK phosphorylates enzymes such as acetyl-CoA carboxylase and HMG-CoA reductase to switch off lipid and protein synthesis and switch on energy-producing pathways, so it is central to metabolism and diabetes research.
| Target | 5'-AMP-activated protein kinase catalytic subunit alpha-1 (gene PRKAA1; UniProt Q13131, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 64.0 kDa |
| Observed MW | 64–66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human HeLa cells, human MCF-7 cells, monkey Cos-7 cells, human K572 cells, human HEK293 cells, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue, mouse intestines tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human AMPK alpha 1/PRKAA1 fragment (Asp359–Pro539), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places 5'-AMP-activated protein kinase catalytic subunit alpha-1 in the cytoplasm, nucleus and late endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yue Zhang et al. (2025) F-53B disrupts energy metabolism by inhibiting the V-ATPase-AMPK axis in neuronal cells. Journal of Hazardous Materials. 10.1016/j.jhazmat.2025.137111
Yanling Tang et al. (2023) PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2023.114772
Jingjing Gong et al. (2026) ACE2 ameliorates DOX-induced cardiotoxicity by suppressing excessive autophagy via the AMPK/mTOR signaling pathway. Biochemical Pharmacology. 10.1016/j.bcp.2026.118119
Jingjing Zhang et al. (2025) Unraveling the role of abnormal AMPK and CRMP-2 phosphorylation in developmental fluoride neurotoxicity: Implications for synaptic damage and neurological disorders. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2025.118192
Anran Lu et al. (2022) The Function behind the Relation between Lipid Metabolism and Vimentin on H9N2 Subtype AIV Replication. Viruses-Basel. 10.3390/v14081814
Lihua Ren et al. (2019) Silica nanoparticles induce spermatocyte cell autophagy through microRNA-494 targeting AKT in GC-2spd cells. Environmental Pollution. 10.1016/j.envpol.2019.113172
Li Yaling et al. (2020) Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy. Frontiers in Pharmacology. 10.3389/fphar.2020.01150
Xinying Zhang et al. (2021) Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway. International Journal of Molecular Medicine. 10.3892/ijmm.2021.4860
Ting Zhang et al. (2022) Fructus Zanthoxyli extract improves glycolipid metabolism disorder of type 2 diabetes mellitus via activation of AMPK/PI3K/Akt pathway: Network pharmacology and experimental validation. Journal of Integrative Medicine-JIM. 10.1016/j.joim.2022.07.004
Xing Yinan et al. (2025) Effect of metformin on airway inflammation in obese asthmatic mice and study of the mechanism involved. Scientific Reports. 10.1038/s41598-025-27977-9
