| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;2.7.11.1 ;Acetyl-CoA carboxylase kinase;ACACA kinase;2.7.11.27 ;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;2.7.11.31 ;Tau-protein kinase PRKAA1;2.7.11.26;PRKAA1;AMPK1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AMPK alpha 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CDD-16 is a rabbit monoclonal antibody that recognizes 5'-AMP-activated protein kinase catalytic subunit alpha-1 (gene PRKAA1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 64.0 kDa, and the supplier reports an observed band at about 64 kDa.
AMPK α1 (PRKAA1) is one of the two catalytic subunits of AMP-activated protein kinase, the cell's main energy sensor. When ATP falls, AMPK phosphorylates enzymes such as acetyl-CoA carboxylase and HMG-CoA reductase to switch off lipid and protein synthesis and switch on energy-producing pathways, so it is central to metabolism and diabetes research.
| Target | 5'-AMP-activated protein kinase catalytic subunit alpha-1 (gene PRKAA1; UniProt Q13131, human) |
|---|---|
| Clone | CDD-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 64.0 kDa |
| Observed MW | 64 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human U-87MG cells, human PC-3 cells, human A549- WT cells, human A549-PRKAA1 KO cells, rat PC-12 cells, rat RH35 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung adenocarcinoma tissue, human tonsil tissue, human pituitary tumor tissue, human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human AMPK alpha 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 5'-AMP-activated protein kinase catalytic subunit alpha-1 in the cytoplasm, nucleus and late endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yue Zhang et al. (2025) F-53B disrupts energy metabolism by inhibiting the V-ATPase-AMPK axis in neuronal cells. Journal of Hazardous Materials. 10.1016/j.jhazmat.2025.137111
Yanling Tang et al. (2023) PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2023.114772
Zhuozhou Hu et al. (2022) Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice. Life Sciences. 10.1016/j.lfs.2022.120725
Anran Lu et al. (2022) The Function behind the Relation between Lipid Metabolism and Vimentin on H9N2 Subtype AIV Replication. Viruses-Basel. 10.3390/v14081814
Bingna Cai et al. (2023) Anti-fatigue effects and mechanism of Syngnathus schlegeli peptides supplementation on exercise-fatigued mice. Journal of Functional Foods. 10.1016/j.jff.2023.105846
Jincheng Yu et al. (2022) Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora. Journal of Functional Foods. 10.1016/j.jff.2022.105255
Xiangxiang Li et al. (2022) Quinacrine attenuates diet-induced obesity by inhibiting adipogenesis via activation of AMPK signaling. European Journal of Pharmacology. 10.1016/j.ejphar.2022.175432
Huang Yuan et al. (2020) Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy. Frontiers in Oncology. 10.3389/fonc.2020.573127
Wencong Ding et al. (2023) Lower dose of metformin combined with artesunate induced autophagy-dependent apoptosis of glioblastoma by activating ROS-AMPK-mTOR axis. Experimental Cell Research. 10.1016/j.yexcr.2023.113691
Lv Yue et al. (2024) Dapagliflozin promotes browning of white adipose tissue through the FGFR1-LKB1-AMPK signaling pathway. Molecular Biology Reports. 10.1007/s11033-024-09540-3
