| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5'-AMP-activated protein kinase subunit beta-1;AMPK subunit beta-1;AMPKb;PRKAB1;AMPK; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AMPK beta 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HFH-16 is a rabbit monoclonal antibody that recognizes 5'-AMP-activated protein kinase subunit beta-1 (gene PRKAB1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 30.4 kDa, and the supplier reports an observed band at about 38 kDa.
AMPK β1 (PRKAB1) is a regulatory subunit of AMP-activated protein kinase that serves as a scaffold joining the catalytic α and nucleotide-binding γ subunits. It carries a glycogen-binding domain and is the site of action of several direct AMPK activators, so it is studied in energy sensing and metabolic drug discovery.
| Target | 5'-AMP-activated protein kinase subunit beta-1 (gene PRKAB1; UniProt Q9Y478, human) |
|---|---|
| Clone | HFH-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.4 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human AMPK beta 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
