| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147). Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects angiogenin in human samples and is validated for ELISA, flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 16.6 kDa, and the supplier reports an observed band at about 17 kDa.
Angiogenin (ANG) is a secreted ribonuclease of the RNase A family that was first identified as an inducer of blood vessel growth. Under stress it cleaves tRNAs in their anticodon loops to produce tRNA-derived fragments that dampen protein synthesis, and ANG variants have been linked to amyotrophic lateral sclerosis.
| Target | Angiogenin (gene ANG; UniProt P03950, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 16.6 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA (capture antibody) | 1–5 µg/mL (Human) |
Samples with a confirmed band (WB): human hepatocellular carcinoma tumor tissue (HCCT).
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human hepatocellular carcinoma tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human Angiogenin/ANG fragment (Gln25–Pro147), expressed in E. coli. Sequence identity with the mouse ortholog: 77.5%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes angiogenin as secreted and also located in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed predominantly in the liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Atri Roozbahani Golnaz et al. (2024) The protein composition of exosomes released by prostate cancer cells is distinctly regulated by androgen receptor-antagonists and -agonist to stimulate growth of target cells. Cell Communication and Signaling. 10.1186/s12964-024-01584-z
Lixin Zhang et al. (2026) A Specific tRNA Half, 3'tiRNA-GlyGCC, Regulates Hypoxic Pulmonary Artery Smooth Muscle Cell Proliferation via Myrf-Mediated Endoplasmic Reticulum Stress. Cell Proliferation. 10.1111/cpr.70238
