| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-1;ANG-1;ANGPT1;KIAA0003; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Angiopoietin 1 recombinant protein (Position: H16-N350). Human Angiopoietin 1 shares 96% amino acid (aa) sequence with both mouse and rat Angiopoietin 1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects angiopoietin-1 (gene ANGPT1) in human samples and is validated for Western blot. The predicted molecular weight is 57.5 kDa, and the supplier reports an observed band at about 65 kDa.
Angiopoietin-1 (ANGPT1) is a secreted ligand that activates the endothelial receptor tyrosine kinase TIE2 (TEK). TIE2 signaling from ANGPT1 promotes endothelial survival and vessel maturation and keeps mature vessels stable and less leaky, so ANGPT1 is studied in angiogenesis, heart development and vascular disease.
| Target | Angiopoietin-1 (gene ANGPT1; UniProt Q15389, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 57.5 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human COLO320 cells, human A549 cells, human HepG2 cells, human 293T cells, human SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Angiopoietin 1 fragment (His16–Asn350), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes angiopoietin-1 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xu Bin et al. (2026) An injectable multi-functional strontium based gentiopicroside hydrogel accelerates bone regeneration via immunomodulation and promoting osteo-/angiogenesis effects. Advanced Composites and Hybrid Materials. 10.1007/s42114-026-01719-5
Chaohui Wang et al. (2026) Rhodiosin promotes cerebral angiogenesis in mice with ischemic stroke via PI3K/Akt pathway activation. Phytomedicine. 10.1016/j.phymed.2026.158247
Ying Li et al. (2024) Salidroside promotes angiogenesis after cerebral ischemia in mice through Shh signaling pathway. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2024.116625
Fengming Tian et al. (2023) Integrating network pharmacology and bioinformatics to explore and experimentally verify the regulatory effect of Buyang Huanwu decoction on glycolysis and angiogenesis after cerebral infarction. Journal of Ethnopharmacology. 10.1016/j.jep.2023.117218
Yuan Yujia et al. (2024) Gut microbiota-derived acetate promotes long-term recovery via angiogenesis guided by lymphatic ingrowth in aged stroke subjects. Frontiers in Neuroscience. 10.3389/fnins.2024.1398913
Ruan Ying et al. (2020) The Changes of Twist1 Pathway in Pulmonary Microvascular Permeability in a Newborn Rat Model of Hyperoxia-Induced Acute Lung Injury. Frontiers in Pediatrics. 10.3389/fped.2020.00190
Ma Jingxi et al. (2008) Effects of Electroacupuncture on Expressions of Angiogenesis Factors and Anti-angiogenesis Factors in Brain of Experimental Cerebral Ischemic Rats after Reperfusion. Journal of Traditional Chinese Medicine. 10.1016/S0254-6272(08)60050-3
