| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-1;ANG-1;ANGPT1;KIAA0003; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin-1, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets angiopoietin-1 (gene ANGPT1) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 57.5 kDa, and the supplier reports an observed band at about 70 kDa.
Angiopoietin-1 (ANGPT1) is a secreted ligand that activates the endothelial receptor tyrosine kinase TIE2 (TEK). TIE2 signaling from ANGPT1 promotes endothelial survival and vessel maturation and keeps mature vessels stable and less leaky, so ANGPT1 is studied in angiogenesis, heart development and vascular disease.
| Target | Angiopoietin-1 (gene ANGPT1; UniProt Q15389, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 57.5 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): recombinant human ANGPT1 protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human angiopoietin-1. It differs from the mouse sequence at three residues and from the rat sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes angiopoietin-1 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
J.F. He et al. (2008) Immunohistochemical and in-situ hybridization study of hypoxia inducible factor-1α and angiopoietin-1 in a rabbit model of mandibular distraction osteogenesis. International Journal of Oral and Maxillofacial Surgery. 10.1016/j.ijom.2008.01.015
X. Ma et al. (2008) Expressions of Angiopoietin-1, Angiopoietin-2, and Tie2 and Their Roles in Rat Renal Allografts With Chronic Allograft Nephropathy. Transplantation Proceedings. 10.1016/j.transproceed.2008.07.121
X. Ma et al. (2008) Rapamycin and Cyclosporine Have Different Effects on Expression of ang-1 and ang-2 and Tie2 in Rat Renal Allograft With Chronic Allograft Nephropathy. Transplantation Proceedings. 10.1016/j.transproceed.2008.08.047
