Anti-Angiopoietin 2/ANGPT2 Antibody

SKU:BHA21000033
Research Validated
—
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Boster Bio
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Overview
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Anti-ANGPT2 antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry. Reactivity: Human. Recognizes angiopoietin-2. Supplied in lyophilized form, ready for use in standard immunodetection workflows. Optimize the working dilution for each application and sample type.
Target ANGPT2
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) IHC
Options selector
Catalog no. Size Conjugation
RP1037 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: IHC
      Application details: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ANGPT2
Alternative names Angiopoietin-2;ANG-2;ANGPT2;
UniProt # O15123
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Immunohistochemistry
Immunogen E.coli-derived human Angiopoietin 2 recombinant protein (Position: Y19-N348). Human Angiopoietin 2 shares 84% and 85% amino acid (aa) sequences identity with mouse and rat Angiopoietin 2, respectively.
Molecular weight 56.9 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) RP1037
Main SKU BHA21000033
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody recognizes angiopoietin-2 (gene ANGPT2) in human samples and is validated for IHC. The predicted molecular weight is 56.9 kDa.

Angiopoietin-2 (ANGPT2) is a TIE2 (TEK) ligand that competes with angiopoietin-1 and often acts as a context-dependent antagonist. It loosens vessel stability, promoting sprouting when VEGF is present and vessel regression when it is not, and it is widely studied in tumor angiogenesis, inflammation and vascular leakage.

Target Angiopoietin-2 (gene ANGPT2; UniProt O15123, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers
Calculated MW 56.9 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 2–5 µg/mL (Human)

Samples with confirmed staining (IHC): human placenta tissue, human adrenal adenoma tissue, human bladder cancer tissue, human glioma tissue, human testis cancer tissue, human rectal cancer tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

Immunogen

Recombinant human Angiopoietin 2 fragment (Tyr19–Asn348), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 84% and 85%, respectively.

Reactivity Notes

The supplier lists reactivity with human. UniProt describes angiopoietin-2 as a secreted protein.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 2–5 µg/mL (Human). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Has this antibody been tested on canine samples?
A.Reactivity with canine samples has not been tested by the supplier. Aligning the immunogen sequence with the canine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Recombinant human Angiopoietin 2 fragment (Tyr19–Asn348), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 84% and 85%, respectively.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Zhang Ji-Hong et al. (2014) Expression of Ang-2/Tie-2 and PI3K/AKT in Colorectal Cancer. Asian Pacific Journal of Cancer Prevention. 10.7314/APJCP.2014.15.20.8651

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