| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-2;ANG-2;ANGPT2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Angiopoietin 2 recombinant protein (Position: Y19-N348). Human Angiopoietin 2 shares 84% and 85% amino acid (aa) sequences identity with mouse and rat Angiopoietin 2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes angiopoietin-2 (gene ANGPT2) in human samples and is validated for IHC. The predicted molecular weight is 56.9 kDa.
Angiopoietin-2 (ANGPT2) is a TIE2 (TEK) ligand that competes with angiopoietin-1 and often acts as a context-dependent antagonist. It loosens vessel stability, promoting sprouting when VEGF is present and vessel regression when it is not, and it is widely studied in tumor angiogenesis, inflammation and vascular leakage.
| Target | Angiopoietin-2 (gene ANGPT2; UniProt O15123, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 56.9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|
Samples with confirmed staining (IHC): human placenta tissue, human adrenal adenoma tissue, human bladder cancer tissue, human glioma tissue, human testis cancer tissue, human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Angiopoietin 2 fragment (Tyr19–Asn348), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 84% and 85%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes angiopoietin-2 as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Ji-Hong et al. (2014) Expression of Ang-2/Tie-2 and PI3K/AKT in Colorectal Cancer. Asian Pacific Journal of Cancer Prevention. 10.7314/APJCP.2014.15.20.8651