| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Formulation | |
| Immunogen | Peptide DNLNATGTNESAFNC corresponding to amino acid residues 21-35 of rat AT2 Receptor (Accession P35351). |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Storage |
Product Overview
Won’t recognize human AT2.
Angiotensin II receptor type 2 or AT2 is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension. Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Gia2 and the Gia3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1. AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others. This antibody can be used in Western blot, immunocytochemistry and immunohistochemistry applications and will recognize AT2 receptor from rat and mouse samples.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | See datasheet |
| Immunohistochemistry (IHC) | See datasheet |
| ICC/IF | See datasheet |
Product Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Isotype | IgG |
| Species Reactivity | Mouse, Rat |
| Purification | Affinity purified on immobilized antigen. |
| Concentration | 1ug/ul |
| Formulation | Liquid |
| Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
Immunogen / Antigen
Peptide DNLNATGTNESAFNC corresponding to amino acid residues 21-35 of rat AT2 Receptor (Accession P35351).
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for WB, IHC, ICC/IF. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Mouse, Rat. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Rabbit. For indirect detection (WB, IHC, IF), select an anti-Rabbit secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Peptide DNLNATGTNESAFNC corresponding to amino acid residues 21-35 of rat AT2 Receptor (Accession P35351).
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Mouse, Rat samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
