| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiotensinogen;Serpin A8;Angiotensin-1;Angiotensin 1-10;Angiotensin I;Ang I;Angiotensin-2;Angiotensin 1-8;Angiotensin II;Ang II;Angiotensin-3;Angiotensin 2-8;Angiotensin III;Ang III;Des-Asp[1]-angiotensin II;Angiotensin-4;Angiotensin 3-8;Angiotensin IV;Ang IV;Angiotensin 1-9;Angiotensin 1-7;Angiotensin 1-5;Angiotensin 1-4;Agt;Serpina8; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse Angiotensinogen, identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects angiotensinogen (gene Agt) in mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 52.0 kDa, and the supplier reports an observed band at about 55 kDa.
Angiotensinogen (AGT) is a liver-derived plasma protein and the sole precursor of the angiotensin peptides. Cleavage by renin and then ACE generates angiotensin II, which constricts blood vessels and stimulates aldosterone release, placing AGT at the start of the renin-angiotensin system that controls blood pressure and fluid balance.
| Target | Angiotensinogen (gene Agt; UniProt P11859, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.0 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
Samples with a confirmed band (WB): mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of mouse angiotensinogen. The peptide sequence is conserved in rat.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes angiotensinogen as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jingwei Yu et al. (2023) Serum exosomes derived from spontaneously hypertensive rats induce cardiac hypertrophy in vitro and in vivo by increasing autocrine release of angiotensin II in cardiomyocytes. Biochemical Pharmacology. 10.1016/j.bcp.2023.115462
Lin Tian et al. (2008) Diabetes-induced upregulation of urotensin II and its receptor plays an important role in TGF-β1-mediated renal fibrosis and dysfunction. American Journal of Physiology-Endocrinology and Metabolism. 10.1152/ajpendo.90672.2008
Tao-Tao Liu et al. (2018) Effects of microRNA-133b on retinal vascular endothelial cell proliferation and apoptosis through angiotensinogen-mediated angiotensin II- extracellular signal-regulated kinase 1/2 signalling pathway in rats with diabetic retinopathy. ACTA Ophthalmologica. 10.1111/aos.13715
