| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ankyrin repeat domain-containing protein 1; Cardiac ankyrin repeat protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear protein; ANKRD1; C193; CARP; HA1A2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ANKRD1 recombinant protein (Position: D120-F319). Human ANKRD1 shares 93.5% and 95% amino acid (aa) sequence identity with mouse and rat ANKRD1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes ankyrin repeat domain-containing protein 1 (gene ANKRD1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
ANKRD1 (also called CARP) is an ankyrin repeat protein expressed mainly in heart and skeletal muscle. It shuttles between the sarcomere and the nucleus, where it can act as a transcriptional co-regulator of cardiac genes, and its expression rises with muscle stress and injury.
| Target | Ankyrin repeat domain-containing protein 1 (gene ANKRD1; UniProt Q15327, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
| Flow cytometry | Not specified by the supplier |
Immunogen
Recombinant human ANKRD1 fragment (Asp120–Phe319), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.5% and 95%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ankyrin repeat domain-containing protein 1 in the nucleus. Tissue expression noted by UniProt (human): Mainly expressed in activated vascular endothelial cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
