| Field | Specification |
|---|---|
| Target | |
| Alternative names | Natriuretic peptides A;CDD-ANF;Cardiodilatin;CDD;Cardiodilatin-related peptide;CDP;Prepronatriodilatin;Atrial natriuretic factor;ANF;Atrial natriuretic peptide;ANP;NPPA;ANP, PND; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ANP, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects natriuretic peptides A (gene NPPA) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 16.4 kDa, and the supplier reports an observed band at about 17 kDa.
Atrial natriuretic peptide (ANP), encoded by NPPA, is a hormone released mainly by atrial cardiomyocytes in response to stretch. It binds the receptor NPR1 to raise cGMP, causing vasodilation, sodium and water excretion and lower blood pressure. ANP is studied in cardiovascular and renal homeostasis and as a marker of cardiac stress.
| Target | Natriuretic peptides A (gene NPPA; UniProt P01160, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 16.4 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|
Samples with a confirmed band (WB): rat cardiac muscle tissue, mouse cardiac muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human ANP. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): [Urodilatin]: Detected in the kidney distal tubular cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ma Yu-kun et al. (2024) Microrna363-5p targets thrombospondin3 to regulate pathological cardiac remodeling. Molecular and Cellular Biochemistry. 10.1007/s11010-024-05125-7
Qinshuo Zhao et al. (2020) Metformin decreased myocardial fibrosis and apoptosis in hyperhomocysteinemia -induced cardiac hypertrophy. Current Research in Translational Medicine. 10.1016/j.retram.2020.103270
