| Field | Specification |
|---|---|
| Target | |
| Alternative names | Natriuretic peptides A; Atrial natriuretic factor prohormone; proANF; Atrial natriuretic peptide prohormone; preproANP; proANP; Atriopeptigen; Cardiodilatin; CDD; preproCDD-ANF; NPPA; ANP; PND |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ANP Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADHD-14 is a rabbit monoclonal antibody that recognizes natriuretic peptides A (gene NPPA) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 16.4 kDa, and the supplier reports an observed band at about 15 kDa.
Atrial natriuretic peptide (ANP), encoded by NPPA, is a hormone released mainly by atrial cardiomyocytes in response to stretch. It binds the receptor NPR1 to raise cGMP, causing vasodilation, sodium and water excretion and lower blood pressure. ANP is studied in cardiovascular and renal homeostasis and as a marker of cardiac stress.
| Target | Natriuretic peptides A (gene NPPA; UniProt P01160, human) |
|---|---|
| Clone | ADHD-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.4 kDa |
| Observed MW | 15 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): PC3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ANP.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): [Urodilatin]: Detected in the kidney distal tubular cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
