| Field | Specification |
|---|---|
| Target | |
| Alternative names | Atrial natriuretic peptide receptor 2; Atrial natriuretic peptide receptor type B; ANP-B; ANPR-B; NPR-B; Guanylate cyclase B; GC-B; NPR2; ANPRB |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ANPRB |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25N77 is a rabbit monoclonal antibody that recognizes atrial natriuretic peptide receptor 2 (gene NPR2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 117.0 kDa, and the supplier reports an observed band at about 117 kDa.
Natriuretic peptide receptor 2 (NPR2, also called GC-B) is a membrane guanylyl cyclase activated by C-type natriuretic peptide (CNP). The resulting cGMP signal is important for endochondral bone growth, and NPR2 mutations cause acromesomelic dysplasia, Maroteaux type. It is also studied in oocyte meiotic arrest.
| Target | Atrial natriuretic peptide receptor 2 (gene NPR2; UniProt P20594, human) |
|---|---|
| Clone | 25N77 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 117.0 kDa |
| Observed MW | 117 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ANPRB.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places atrial natriuretic peptide receptor 2 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Peng Hu et al. (2016) Neutral endopeptidase and natriuretic peptide receptors participate in the regulation of C-type natriuretic peptide expression in renal interstitial fibrosis. Journal of Receptors and Signal Transduction. 10.3109/10799893.2016.1155068
