| Field | Specification |
|---|---|
| Target | |
| Alternative names | Antithrombin-III;ATIII;Serpin C1;SERPINC1;AT3;PRO0309; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Antithrombin III, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects antithrombin-III (gene SERPINC1) in rat, mouse and human samples and is validated for Western blot. The predicted molecular weight is 52.6 kDa, and the supplier reports an observed band at about 57 kDa.
Antithrombin-III (antithrombin, SERPINC1) is the main plasma serpin that controls blood coagulation. It inactivates thrombin and factors IXa, Xa and XIa, and its activity is strongly accelerated by heparin, which is the basis of heparin's anticoagulant effect; inherited antithrombin deficiency increases the risk of venous thrombosis.
| Target | Antithrombin-III (gene SERPINC1; UniProt P01008, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 52.6 kDa |
| Observed MW | 57 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
|---|
Samples with a confirmed band (WB): rat testis tissue, SMMC cells, Jurkat cells, RAJI cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of human antithrombin III. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt describes antithrombin-III as a secreted protein. Tissue expression noted by UniProt (human): Found in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shiwen Wang et al. (2024) Circulating exosomal protein EFEMP1 and SERPINC1 as diagnostic biomarkers for epithelial ovarian cancer. Translational Oncology. 10.1016/j.tranon.2024.102126
Mori Saaya et al. (2017) A novel frameshift mutation leading to inherited type I antithrombin deficiency. Blood Coagulation & Fibrinolysis. 10.1097/MBC.0000000000000555
Ikeda Yuichi et al. (2019) Safe childbirth for a type 1 antithrombin-deficient woman with novel mutation in the SERPINC1 gene undergoing antithrombin concentrate therapy. Blood Coagulation & Fibrinolysis. 10.1097/MBC.0000000000000785
