| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptotic protease-activating factor 1;APAF-1;APAF1;KIAA0413; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human APAF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BHF-1 is a rabbit monoclonal antibody that recognizes apoptotic protease-activating factor 1 (gene APAF1) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 141.8 kDa, and the supplier reports an observed band at about 142 kDa.
Apoptotic protease-activating factor 1 (APAF1) is the central scaffold of the apoptosome. When cytochrome c is released from mitochondria, APAF1 binds it and ATP/dATP, oligomerizes, and activates caspase-9, which then activates caspase-3 to execute the intrinsic pathway of apoptosis.
| Target | Apoptotic protease-activating factor 1 (gene APAF1; UniProt O14727, human) |
|---|---|
| Clone | BHF-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 141.8 kDa |
| Observed MW | 142 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): mouse kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human APAF1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places apoptotic protease-activating factor 1 in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
