| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA- (apurinic or apyrimidinic site) lyase;3.1.-.-;4.2.99.18;APEX nuclease;APEN;Apurinic-apyrimidinic endonuclease 1;AP endonuclease 1;APE-1;REF-1;Redox factor-1;DNA- (apurinic or apyrimidinic site) lyase, mitochondrial;APEX1;APE, APE1, APEX, APX, HAP1, REF1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA repair nuclease/redox regulator APEX1 in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 35.6 kDa, and the supplier reports an observed band at about 36 kDa.
APEX1 (also called APE1 or Ref-1) is the main apurinic/apyrimidinic endonuclease of the base excision repair pathway, cutting DNA at abasic sites created by oxidative and alkylating damage. It also acts as a redox regulator that activates transcription factors such as AP-1, NF-κB and p53, making it central to research on DNA repair and oxidative stress.
| Target | DNA repair nuclease/redox regulator APEX1 (gene APEX1; UniProt P27695, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 35.6 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human K562 cells, human HepG2 cells, human Raji cells, human A431 cells, human A549 cells, human MCF-7 cells, human RT4 cells, human U87 cells, rat C6 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with confirmed staining (ICC/IF): human colon cancer tissue.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human APE1 fragment (Pro2–Leu318), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94% and 93%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA repair nuclease/redox regulator APEX1 in the nucleus, nucleus speckle, endoplasmic reticulum and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hong-qiang Chen et al. (2020) Epigenetic silencing of TET1 mediated hydroxymethylation of base excision repair pathway during lung carcinogenesis. Environmental Pollution. 10.1016/j.envpol.2020.115860
