| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA- (apurinic or apyrimidinic site) lyase 2;3.1.-.-;4.2.99.18;AP endonuclease XTH2;APEX nuclease 2;APEX nuclease-like 2;Apurinic-apyrimidinic endonuclease 2;AP endonuclease 2;APEX2;APE2, APEXL2, XTH2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human APEX2 recombinant protein (Position: M1-G500). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA-(apurinic or apyrimidinic site) endonuclease 2 (gene APEX2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 57.4 kDa, and the supplier reports an observed band at about 57 kDa.
APEX2 (APE2) is a second apurinic/apyrimidinic endonuclease with weaker AP site incision activity than APEX1 but notable 3′-5′ exonuclease activity. It takes part in base excision repair and in responses to oxidative DNA damage and replication stress.
| Target | DNA-(apurinic or apyrimidinic site) endonuclease 2 (gene APEX2; UniProt Q9UBZ4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 57.4 kDa |
| Observed MW | 57 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SH-SY5Y cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human APEX2 fragment (Met1–Gly500), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA-(apurinic or apyrimidinic site) endonuclease 2 in the nucleus, cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Highly expressed in brain and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
