| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin-like protein ATG12;Autophagy-related protein 12;APG12-like;ATG12;APG12, APG12L; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Apg12(Atg12) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17A34 is a rabbit monoclonal antibody that recognizes ubiquitin-like protein ATG12 in human samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 15.1 kDa; the supplier reports observed bands at 22, 55 kDa.
ATG12 is a ubiquitin-like protein that is covalently joined to ATG5 by the enzymes ATG7 and ATG10. The resulting ATG12-ATG5 conjugate, together with ATG16L1, promotes lipidation of LC3 family proteins and is essential for autophagosome formation.
| Target | Ubiquitin-like protein ATG12 (gene ATG12; UniProt O94817, human) |
|---|---|
| Clone | 17A34 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 15.1 kDa |
| Observed MW | 22, 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human apg12(Atg12).
Reactivity Notes
The supplier lists reactivity with human. UniProt places ubiquitin-like protein ATG12 in the cytoplasm and preautophagosomal structure membrane. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Na Zhao et al. (2022) DJ-1 activates the Atg5-Atg12-Atg16L1 complex via Sirt1 to influence microglial polarization and alleviate cerebral ischemia/reperfusion-induced inflammatory injury. Neurochemistry International. 10.1016/j.neuint.2022.105341
