| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin-like-conjugating enzyme ATG3;6.3.2.-;Autophagy-related protein 3;APG3-like;hApg3;Protein PC3-96;ATG3;APG3, APG3L; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Apg3 (Atg3) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABBC-1 is a rabbit monoclonal antibody that recognizes ubiquitin-like-conjugating enzyme ATG3 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 35.9 kDa, and the supplier reports an observed band at about 40 kDa.
ATG3 is the E2-like conjugating enzyme that attaches ATG8 family proteins, such as LC3 and GABARAP, to phosphatidylethanolamine. This lipidation step anchors LC3 to the growing autophagosome membrane and is essential for autophagy.
| Target | Ubiquitin-like-conjugating enzyme ATG3 (gene ATG3; UniProt Q9NT62, human) |
|---|---|
| Clone | ABBC-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.9 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human Jurkat cells, human K562 cells, human Raji cells, monkey COS-7 cells, rat C6 cells, mouse kidney tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human apg3 (Atg3).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin-like-conjugating enzyme ATG3 in the cytoplasm. Tissue expression noted by UniProt (human): Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jing-jing Xing et al. (2019) Ginsenoside Rb3 provides protective effects against cisplatin-induced nephrotoxicity via regulation of AMPK-/mTOR-mediated autophagy and inhibition of apoptosis in vitro and in vivo. Cell Proliferation. 10.1111/cpr.12627
Yang S. J. et al. (2024) Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro. Molecular Biology. 10.1134/S0026893324700109
