| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gamma-secretase subunit APH-1A;APH-1a;Aph-1alpha;Presenilin-stabilization factor;APH1A;PSF;CGI-78, UNQ579/PRO1141; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human APH1a, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes gamma-secretase subunit APH-1A (gene APH1A) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 29.0 kDa, and the supplier reports an observed band at about 29 kDa.
APH1A is a non-catalytic subunit of γ-secretase, the intramembrane protease complex that also contains presenilin, nicastrin and PEN-2. It is needed for complex assembly and stability, and γ-secretase cleaves substrates such as Notch receptors and the amyloid precursor protein, so APH1A is studied in Notch signaling and Alzheimer's disease.
| Target | Gamma-secretase subunit APH-1A (gene APH1A; UniProt Q96BI3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 29.0 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human U87 cells, human U251 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human APH1a. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places gamma-secretase subunit APH-1A in the endoplasmic reticulum membrane and Golgi apparatus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Joshua Abd Alla et al. (2026) Analysis of GRK2 aggregation in the pathology of Alzheimer disease in animal models. Cell Reports Medicine. 10.1016/j.xcrm.2026.102707
